Normalizing phosphoproteomics data
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5 weeks ago
mm2568 • 0

Hi! I am trying to normalize phosphoproteomics data before doing a differential expression analysis (calculate fold changes and p-values) using ROTS* for 2 cell lines (4 replicates for the first and 3 replicates for the second)

ROTS documentation

In the package they recommend to normalize the data before by using "standard normalization techniques", but I am not sure what standard techniques are.

I have a dataset with the first column being the "gene and the phosphorylation site" and then seven columns following with the abundance for both cell lines in seven columns total.

I would appreciate your guidance on this simple issue! Thank you!

R phosphoproteomics normalize • 103 views
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