How to assign A-site for a ribosome fragments obtained from ribosome sequencing data of E. coli
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2.5 years ago

Ribosome typically covers ten codons, and the A-site is found to be at the 6th codon position. However, due to the stochastic cleavage/digestion, different lengths of fragments can arise. So, in this case, assigning A-site at the 6th codon position can lead to an erroneous result.

I have gone through the literature, and I did not find a way to assign A-site to the ribosome protected fragments in the case of E. coli.

I will be thankful if you provide me with any help

data sequencing ribosome assignment A-site • 398 views
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