Dear Seniors and All members,
I am wondering whether anyone has done weighted gene co-expression network analysis (WGCNA) from Bulk-RNAseq before. I followed the WGNCA tutorial using my data and the module detection outputs are here.
However, once I visualized the modules in clustering, I got weird output like the following
I have attached the clustering of modules from the tutorial, which looks nice and interpretable. I dont expect my data to be the same like this, but just would like to check whether I have done anything wrong with my code.
I have gone through identifying the soft-threshold power and following are the code chuncks I used. Wondering whether this is due to my data or might be sth wrong or missing there. Really appreciate any help from member who have done it before or anyone can help is welcome.
Thank in advance.