Entering edit mode
2.5 years ago
gudetama2001
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0
Hello,
I am a beginner in this field and some days ago I thought of detecting circRNAs from the RNA-seq data. I know that circRNA transcripts have somewhat "peculiar" order: "5’ exons were ‘shuffled’ downstream of 3’ exons. Despite the non-canonical ordering, the exons were complete and used the usual splice donor and acceptor sites." (Jeck et al. 2014).
My question is: why do we need an extra tool for the circRNA identification like find_circ? Why can't normal alignment algorithms detect circRNAs, too?
Thank you-