Entering edit mode
2.4 years ago
Jiejing
•
0
hi, I'm fairly new to metagenome. when I run kneaddta to control quelity, I got error from bowtie2. my command:
kneaddata -i 1-1103_FDSW202359621-1r_1.fq -i 1-1103_FDSW202359621-1r_2.fq -o ./ -v -t 8 --remove-intermediate-output --trimmomatic /home/wangjiejing/miniconda3/envs/qc/share/trimmomatic/ --trimmomatic-options "ILLUMINACLIP:/home/wangjiejing/miniconda3/envs/qc/share/trimmomatic/adapters/TruSeq2-PE.fa:2:40:15 SLIDINGWINDOW:4:20 MINLEN:50" --reorder --bowtie2-options "--very-sensitive —-dovetail" -db /home/wangjiejing/db/kneaddata/human_genome/Homo_sapiens
Error :
Error message returned from bowtie2 :
Extra parameter(s) specified: "—-dovetail"
Note that if <mates> files are specified using -1/-2, a <singles> file cannot
also be specified. Please run bowtie separately for mates and singles.
Error: Encountered internal Bowtie 2 exception (#1)
Command: /home/wangjiejing/miniconda3/envs/qc/bin/bowtie2-align-s --wrapper basic-0 --threads 8 -x /home/wangjiejing/db/kneaddata/human_genome/Homo_sapiens -S /data/wangjj/metagenomics_tutorial/seq_test/_temp.samoh4gigkneaddata_ --no-head --very-sensitive --phred33 --reorder -1
/data/wangjj/metagenomics_tutorial/seq_test/1-1103_FDSW202359621-1r_1_kneaddata.trimmed.1.fastq -2 /data/wangjj/metagenomics_tutorial/seq_test/1-1103_FDSW202359621-1r_1_kneaddata.trimmed.2.fastq -U /data/wangjj/metagenomics_tutorial/seq_test/1-1103_FDSW202359621-1r_1_kneaddata.trimmed.single.1.fastq,/data/wangjj/metagenomics_tutorial/seq_test/1-1103_FDSW202359621-1r_1_kneaddata.trimmed.single.2.fastq —-dovetail
(ERR): bowtie2-align exited with value 1
I have the similar error, but from kneaddata_bowtie2_discordant_pairs. I don't know how to solve this. It worked fine until a few months ago. But something went wrong yesterday.