Increasing biological replicate count to perform pseudobulk differential expression analysis

Question

[Ipotco]

Hi,

I have 10X Visium data (mouse) for 4 different conditions. Unfortunately, only 3 of the 4 conditions have multiple biological replicates (3x replicates each), while the remaining condition (the control) consists of only a single replicate.

I would like to perform a pseudobulk differential expression analysis. Is there a robust statistical method for simulating pseudo replicates that would allow me to increase the number of replicates in my control group? If not, what would you suggest as an alternative?

Thanks in advance!