Non-zero return code 127 from Needle

Question

[Park]

I am struggling with running Needle. What did I do wrong?

from Bio.Emboss.Applications import NeedleCommandline
needle_cline = NeedleCommandline("/opt/anaconda3/envs/SmartBioCode/bin/needle", asequence="needle1.fasta", bsequence="needle2.fasta", gapopen=10.0, gapextend=1.0, outfile="needle.txt") print(needle_cline.outfile)
stdout, stderr = needle_cline() print(stdout + stderr)
ApplicationError: Non-zero return code 127 from '/opt/anaconda3/envs/SmartBioCode/bin/needle -outfile=needle.txt -asequence=needle1.fasta -bsequence=needle2.fasta -gapopen=10.0 -gapextend=1.0', message 'usage: dirname string [...]'

[Alex Reynolds]

Please past text of your error message, not an image. The contents of an image cannot be indexed for their textual content and that makes it harder for people to come back to this question later on.