Entering edit mode
10 months ago
Maria17 ▴ 10
As part of my qPCR analysis, I performed two qPCR assays for the same samples using two different protocols. I have two data frames with rows of samples and columns of RNAs. The rows and columns have the same names in both data frames.
I don't know how I can compare each CT value between the two data frames shown in a barplot or other plot.
I would appreciate any solution or recommendation.
I don't think you have a clear idea of the experimental goals of your qPCR experiment so I'd recommend taking a step back. Are you trying to compare the results from the two different protocols? Are the two conditions done in a similar enough way to even compare? Are you planning on doing dCt or ddCt?
it is the dCt, i want to compare pPCR output of two different protocol ,the samples are the same. basically comparing the number of dCt from two protocol & plotting in a bar graph or correlation graph.