DTU by combining different type of RNA seq data
1
0
Entering edit mode
7 weeks ago
aimanbarki ▴ 20

Hello , I am working with some human data from ncbi. I was able find 5 (2 controls + 3 other group) samples which are unstranded polyA plus and 4 ((2 controls + 32other group) samples are Total RNA – Strand-specific (reverse). Is there any way I can use them in one DTU analysis (Hisat2- DEXSeq) or if I used them what are the precautions I need to take?

DTU analysis • 181 views
ADD COMMENT
0
Entering edit mode
7 weeks ago
Trivas ▴ 480

I think you could implement batch correction (RUV, combat) to account for sample prep differences.

ADD COMMENT

Login before adding your answer.

Traffic: 1745 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6