Replicated samples analysis
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6 weeks ago
valentinavan ▴ 40

Hi,

I have biological replicates: they come from the same raw sample (tube of sediment) but from different DNA extraction & sequencing experiments. These are metagenomes and I used whole genome sequencing.

If I treat them separately, the abundances are consistent, some of them cluster together on a PCoA but not so well on hclust.

Question:

1) Should I concatenate the raw fastq files together beforehand?

2) Or should I treat them separately until after the alignment to a db step and then sum the counts of each replicates together?

What is the best procedure?

Thanks

repeats replicates • 161 views
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