Within-sample comparison of gene expression and replicates
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22 months ago
msrk04011 • 0

Hello, I have a question related to a within-sample comparison of the expression of genes. I have a set of triplicated RNA-Seq datasets. I would like to compare the expression of gene X1, X2, X3, ... and X10 within the samples. I ran StringTie with each samples (with merged GTF) and obtained GTF files with TPM values for each replicate samples. So I can compare the expression of the genes within each samples. Then, is it OK to average the TPM values for each gene from the triplicate samples, to make the analysis as "replicated"? This may be a kind of very basic question, but I will be grateful if anyone give me the answer. Thank you so much.

RNA-Seq • 331 views
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