I referred paper named 'CRISPR-Cas9-Based Discovery of the Verrucosidin Biosynthesis Gene Cluster in Penicillium polonicum' to find out certain gene cluster from my genomic analysis data.
They utilized antiSMASH to get gene cluster and I conducted same process, but I couldn't find same gene cluster, and I found out another process.
'To identify BGCs in the genomes of P. polonicum strain IBT4502 (GCA_002072265.1) and P. polonicum strain hy4 (GCA_003344595.1), antiSMASH (Weber et al., 2015) was used and only clusters containing a putative PKS similar to both CtvA protein (Q0C9L7.1) and AurA (A0A0M4L8I7.1) were further considered (query coverage ≥50% and e-value > e–5).'
They run antiSMASH and additionally found gene clusters which contain certain protein sequence (CtvA and AurA).
Those antismash can make it, or another program is required?
They probably used blast or similars to search for CtvA and AurA homologs only in PKS gene clusters.