Entering edit mode
20 months ago
ingerslev
•
0
How does bcl-convert handle reads with an UMI in the 3' end and adapter sequences? The bcl-convert documentation is unclear on the order.
If I set the SampleSheet to contain:
[BCLConvert_Settings]
AdapterRead1,AGATCGGAAGAGCACACGTCTGAACTCCAGTCA
OverrideCycles,I8;Y48U4
Are the adapters trimmed before UMIs are extracted?
Also for convenience, Is there any way to specify something like Y*U4
to mean everything except the last 4 bases?