How do I get estimated gene counts for a reference that contains GFP using Kallisto?
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21 months ago
bioinfo ▴ 150

Hello,

I am interested in aligning some samples to a human-GFP reference using kallisto. From what I understood to make the new kallisto reference I just need to concatenate the human cDNA fasta file with the GFP fasta file and then create the index with kallisto. However, I am not sure how to produce the gene counts afterwards. Normally, following allignment I use tximport and the ensembl package (through Biomart) to generate counts. Should I add GFP in the tx2gene dataframe that contains the transcript and gene IDs?

Thank you

gene ensembl tximport kallisto • 382 views
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