high throughput drug screening
0
0
Entering edit mode
2.2 years ago
Ahmed.waraky ▴ 10

Hi Few questions on HTS drug screening.

Does anyone know a pipeline/workflow available for HTS from quality control, to normalization and Hit identification?? If not, any R-library for data analysis??

When obtaining the raw intensity data, Should I plot for normal probability first in order to decide which normalization method (Z-score, B-score) to use?

In case of normal distribution, and in case of low variability between the controls. Should I use normalized percent inhibition or Z-score for the normalization method?

In case of normalized percent inhibition, do I use all controls in all plates?? or each plate is normalized to its controls in the same plate??

Thanks!

screening HTS throughput drug high • 366 views
ADD COMMENT

Login before adding your answer.

Traffic: 1305 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6