News:course: RNA-seq analyses in non-model organisms
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19 months ago
carlopecoraro2 ★ 2.5k

Dear all,

there are the last 5 seats available for the 3rd edition of the course: "RNA-seq analyses in non-model organisms"

Dates: online, 28th of November - 2nd of December 2022

This workshop provides an overview of modern applications of transcriptome sequencing and popular tools and algorithms for exploring transcript reconstruction and expression analysis in a genome-free manner, leveraging the Trinity software and analysis framework. Attendees will perform quality assessment of Illumina RNA-Seq data, assemble a transcriptome using, among others, Trinity, quantify transcript expression, leverage Bioconductor tools for differential expression analysis, and apply Trinotate to functionally annotate transcripts. In parallel to the short-read assembly, participants will perform the pre-processing of 3rd generation sequencing data (PacBio IsoSeq) and the resulting assemblies will be compared. Additional methods will then be explored for characterizing the assembled transcriptome and revealing biological findings.

differential Bioconductor RNAseq expression non-model organisms analysis R • 417 views

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