Entering edit mode
18 months ago
glyptonotus
▴
20
Hi,
I would like to know how can I introduce two different labels in --refPointLabel when constructing a heatmap using different BED files from different regions.
I have 3 BigWig files (3 samples) and 2 BED files (one with TSS coords and the other one with TTS coords) and I have perfomed the following steps:
computeMatrix reference-point -S sample1.bw sample2.bw sample3.bw\
-R BED_TSS BED_TTS
-b 4000 -a 4000 \
--missingDataAsZero --maxThreshold 1000 --skipZeros --sortRegions keep
-o output.mat.gz
plotHeatmap -m output.mat.gz --refPointLabel 'TSS TTS' --perGroup --sortUsingSamples 1 2 3 -out output.png
However, I cannot use --refPointLabel 'TSS TTS' since only one entrance is permitted. In the following picture, left heatmaps correspond to the TSS region and right ones to the TTS. How could I solve this to plot TSS one the the left maps and TTS on the right ones?
Thanks for any suggestion!