Sorry if this has been asked multiple times before - I’m wondering what’s possible in the current landscape of bioinformatics tools.
I’m planning on sequencing a set of 200,000 cells or so using 10X’s fixed RNA profiling technology. I’d like to at least try to analyze the data myself. My main device has 16 cores and 64gb RAM - wondering how doable that is, or if I need to look into other solutions. I’m not a fan of HPC because I don’t like submitting jobs into a queue…I prefer getting results back in real time if possible and tracking progress.
Any thoughts? Feel free to tell me this plan makes no sense!