no strand information on miRNome
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9 days ago
diogo.moraes ▴ 10

Hello

I am currently analyzing differentially expressed miRNas of an experiment. However, there is no strand information on the data I was given. I was not the person who aligned it, so I am not sure of how it was done. I have only worked with regular RNA-seq data before, and as this was never necessary to me, I have no idea if it is possible to align it while keeping the strand information. To check their predicted targets I would need to know exactly which strand is upregulated. Is there any work around this, or would you recommend aligning it again while keeping the strand information somehow?

mirna mirnome rna-seq • 88 views
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