I am currently analyzing differentially expressed miRNas of an experiment. However, there is no strand information on the data I was given. I was not the person who aligned it, so I am not sure of how it was done. I have only worked with regular RNA-seq data before, and as this was never necessary to me, I have no idea if it is possible to align it while keeping the strand information. To check their predicted targets I would need to know exactly which strand is upregulated. Is there any work around this, or would you recommend aligning it again while keeping the strand information somehow?