I recently updated the indexes I am using for kallisto from version 93 to 96. I tried to create genomebam files and I used the same chromosome file I was using in the past. However, now I am getting the following warning:
Warning: could not find chromosomes for 491 transcripts Warning: 25039 transcripts were defined in GTF file, but not in the index
Does that mean that I need to find a new chromosome file? Where can I find a recent one? I am interested in GRCm38. The one we had on file is from 2016. I am using the kallisto index and gtf file from the prebuilt indexes in kallisto.
I am using kallisto 0.44.0. I noticed that some people were having that issue with kallisto 0.46.2 (https://github.com/pachterlab/kallisto/issues/254). I really don't want to change the kallisto version unless I absolutely have to. I also noticed that the chromosome file that I have is the same as the one mentioned in the ticket.
Thank you for replying. You said that this should shouldn't really make a difference. Does that mean that I can still use the BAM files that were generated?
Thank you for your help.