Entering edit mode
20 months ago
kirillkirilenko
▴
40
Hello! We sequenced mosquitoes gut using SQK-16S024 kit on MIN106 flowcell. Mean Q-score is about 11, Mode Q-score is about 10. Now we want to analyse bacterial composition in mosquitoes using EPI2ME Fastq 16S tool. I want to know the following: Fastq 16S uses BLAST for compositional analysis therefore if you were to set some BLAST metrics on your own, which percentage of the BLAST identity (which is 77% by default) would be acceptable based on our Q-score? Are there any suggestions? I appreciate it in advance!
