maximum fastq file size in galaxy?
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13 months ago

Hello I got a couple of miRNA-seq fastqs and I uploaded them on galaxy as a paired collection. One of my fastqs, however, is much larger than the others, as they range from 600mb to 2.4gb, but one sample has 27gb. When I turn those files into a collection, the 27gb pair becomes 4gb. Is the only solution to break down this file in multiple ones to process it? Will I have multiple count matrices for the same sample when I align it and then I should just sum up the counts?

fastq rna-seq usegalaxy • 337 views

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