Question

FASTQC RESULTS

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2.7 years ago

mshayat • 0

$should i proceed to assembly ?![\]\[1\]$

fastqc • 815 views

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What to do with perbase sequence content and sequence duplication levels?

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what to do with per base sequence content and sequence duplication levels?

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score 1 · Answer 1 · 2023-02-13

There is no rule that says that every FastQC category needs to be Green before you can proceed with further analysis. FastQC results need to be taken in context of the experiment being done since the limits that are tested (which generate the pass/fail flags) are for normal genomic sequencing.

Please check our several blog posts from FastQC authors that should address most of your questions: https://sequencing.qcfail.com/software/fastqc/

Following two address:

Per base content: https://sequencing.qcfail.com/articles/positional-sequence-bias-in-random-primed-libraries/
Sequence duplication: https://sequencing.qcfail.com/articles/libraries-can-contain-technical-duplication/