Entering edit mode
2.7 years ago
KKH
•
0
Hello. I am a 1st year grad student in genetics lab. I am new to bioinformatics.
computer: MS-Linux ubuntu-miniconda3
data: fish tissue gDNA NGS(MGISEQ,, 150 paried end read) picard bam, bai file
My goals are
- Compare sample seq with NCBI reference seq and find deletion sites(over 50bp) and download the result file containg site positon and deletion sequence
- There are 22 chromosome bam file. I want to get each chromosome fasta file. (not 150bp multiple fasta but one whole aligned fasata file)
Which sv tools can I use? GATK? freebayes? Mutect2? delly?
And can you recommend some tutorial site or video?
Thank you so much for your attention.