RNAseq data from patients from 2 time points (before and after treatment) and want to do gene expression analysis comparing 2 time points.
if I want to use
DESeq2, how should I make my
metadata.csv file which will be used for differential gene expression analysis.
normally I make the
metadata.csv file like this:
and at the end I will get fold change and p-values. how I should make this file for my data to compare 2 time points? shall I do it per individual or include all patients in the same