PISCES - VARIANT ALLELE FREQUENCY - COMMAS
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2.3 years ago
accdg2022 • 0

Hello,

Why when I run PISCES (after getting the realigned BAM file with GEMINI) does it report the VAFs with commas instead of dots? In this example, it should report 0.55. I have already check that it was not a bug from my text editor by opening it with vim:

GT:GQ:AD:DP:VF:NL:SB 0/1:43:155,9:164:0,55:20:-14,2244

The code used would be:

bashArguments = "Pisces -bam "+bamGemini+" -g "+refGenomeFolder+" -i "+targetBedFileIntervalList+" -UseStitchedXD TRUE -CallMNVs TRUE -MinBQ 30 -MinVF 0. 001 -MinMQ 10 --OutFolder "+outDir+"/pisces/pisces/"+sample+" --MaxNumThreads "+cpus

Thank you very much!

PISCES MUTATION VAF VARIANT • 499 views
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