How to specify which resolution I want to be plotted in the UMAP?
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9 months ago
camillab. ▴ 160

Hi,

Maybe is it a stupid question but I integrated two dataset with harmony and like this post I tried different resolutions. How can I specify which resolution I want to be plotted? Dimplot show only the last resolution used (38 cluster - In my opinion too many). last resolution

I tried following tutorial but when I do

Idents(object = harmonized_seurat) <- "integrated_snn_res.0.2"

I get the cells distributed in the umap but not divided in clusters (eg. it should be 16 clusters).enter image description here

This part of my integrated dataset when I do harmonized_seurat@meta.data %>% View() enter image description here

Thank you!!!!

Camilla

resolution scRNSeq harmony R map • 812 views
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When you call Idents(), you should assign a vector corresponding to the clustering identifiers of samples, not a single character

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