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2.2 years ago
vusichj
•
0
I am having the same problem as this post: Trouble with bedtools getfasta
When I run the following command, I get this warning for every chromosome and the output fasta is empty:
bedtools getfasta -fi GRCm38.p6.genome.fasta -bed e2f5_R1.seacr.peaks.stringent.bed -fo e2f5_R1.seacr.peaks.fasta
WARNING. chromosome (1) was not found in the FASTA file. Skipping.
However, here is the result of running the following code:
file GRCm38.p6.genome.fasta
GRCm38.p6.genome.fasta: ASCII text
My bed file head:
1 6213822 6215942 291.2 0.492455 1:6214772-6214794
1 9629223 9632268 339.898 0.346799 1:9630315-9630327
1 9699289 9701242 274.547 0.388415 1:9700690-9700691
1 13371903 13374929 340.765 0.575686 1:13372731-13372736
1 16103645 16107372 638.665 0.645046 1:16105565-16105568
1 23255565 23257926 358.188 0.679726 1:23256351-23256352
1 34438613 34440337 361.67 0.957165 1:34439596-34439625
1 36307174 36310195 383.227 0.582622 1:36307847-36307911
1 36469694 36472463 379.391 0.547943 1:36471579-36471580
1 36938872 36940595 357.647 1.08895 1:36939697-36939840
Thanks in advance if you are able to help.