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9 months ago
1595952559
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Hello, everyone, recently when I run the megalodon command, there was a constant error. attached is my demo in the script.
megalodon \
~/01.data/01.ONT_data/02.ONT_test/AW/FAST5_PASS \
--guppy-params "-d /public/home/zenglingsen/04.software/03.Guppy/rerio/basecall_models/" \
--guppy-server-path /public/home/zenglingsen/04.software/03.Guppy/ont-guppy/bin/guppy_basecall_server \
--guppy-config res_dna_r941_prom_modbases_5mC_CpG_v001.cfg \
--outputs basecalls mappings mod_mappings mods per_read_mods \
--reference /public/home/zenglingsen/01.data/03.Reference/GCF_000003025.6_Sscrofa11.1_genomic.fna \
--mod-motif m CG 0 \
--output-directory /public/home/zenglingsen/01.data/01.ONT_data/02.ONT_test/AW/FAST5_PASS/out \
--overwrite \
--devices 0 \
--processes 8 \
and this is the log file.
2023-08-08 10:17:01.473438 [guppy/message] ONT Guppy basecall server software version 6.0.1+652ffd1, client-server API version 10.0.0
log path: /public/home/zenglingsen/01.data/01.ONT_data/02.ONT_test/AW/FAST5_PASS/out/guppy_log
chunk size: 2000
chunks per runner: 512
max queued reads: 2000
num basecallers: 4
num socket threads: 2
max returned events: 50000
gpu device: cuda:1
kernel path:
runners per device: 4
2023-08-08 10:17:01.475945 [guppy/info] crashpad_handler not supported on this platform.
2023-08-08 10:17:01.478127 [guppy/info] Listening on port ipc:///tmp/3763-2667-29cb-f0a3.
2023-08-08 10:17:01.607464 [guppy/error] CUDA error at /builds/ofan/ont_core_cpp/ont_core/common/cuda_common.cpp:232: CUDA_ERROR_INVALID_DEVICE.
Error initialising basecall server using port: ipc://auto. Aborting.
2023-08-08 10:17:01.608638 [guppy/message] The basecall server has shut down successfully.
this is the GPU info:
Now I have no choice but to ask for your help, any advice and comment would be appropriate and helpful. Thank you.