How to combine only reads columns from 6 different NM transcript ID based quant.sf files
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2.1 years ago
Sacit • 0

Hello,

Being beginner, I want to just know what is the simplest way to combine only read columns from 6 different NM transcript based quant.sf files. These transcript belongs to sheep and I want to do DE analysis.

I can provide further information, if need to answer this question.

Salmon RNA-Seq • 629 views
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Entering edit mode
2.1 years ago
ATpoint 89k

I assume you use salmon, you have now transcript-level abundances and want gene-level abundances? If so, use the standard package for this summarization which is https://bioconductor.org/packages/release/bioc/html/tximport.html

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