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langziv
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Hello
How can one do such analysis without assembling the genome? Is it possible?
Hello
How can one do such analysis without assembling the genome? Is it possible?
Yes, you most certainly can do this analysis. Contig-level assemblies may have more duplicated genes not collapsed into a single locus, but otherwise should be fine. If the assembly was released in a study, you can check how complete the transcriptome is estimated to be.
You can do one of these analyses following any of the numerous tutorials available online.
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Yes, it is possible and there are a few was to go and tackle the problem. The one that might be most convenient for you is doing a de-novo transcriptome assembly. There are a few programs that can do this: