WGNCNA , hub gene identification

Question

[Mehvi khan]

Hello All,

I have RNASeq analysis data , of three parental cell lines and out of which three resistant isomorphic celline are derived . I have performed the RNAseq analysis and now want to do WGCNA. I have created the modules without the traits . I want to identify the the top 10 hub genes in modules . I have some quick questions : Q1) what is the trait file , like what is needed in trait file ? how to design it Q2) If I dont have the trait files how can I identify the important genes in my data ?

Please Any help will be appreciated or any good resource to read.

Thankyou Mehwish

Answer 1

[Mehvi khan]

The following is the color coded modules that I created from a blog

I am a little stuck in its interpretation and all.. and how can I find what genes are in which module