I am new to analysis of Flow Cytometry data, and am trying to get an understanding of the overall workflow from data preparation to some kind of dimension reduction visualization. During the transformation step, the arcsinh function is often applied to the data. This transformation is dependent on the cofactor parameter (which has defaults of 5 for multicolor flow, and 150 for normal flow cytometry (FC)).
In my googling, I have only found the concept of the cofactor to come up in discussions related to flow cytometry. Does that make sense?