Algorithmic Solutions for Resolving Overlapping Sequences in Sanger Sequencing of PCR Segments

Question

[Amior]

Hello, I've encountered a challenge in my Sanger sequencing data for a PCR segment, where I have mixed primers on both sides, resulting in overlapping sequences. I'd like to explore algorithmic approaches to separate and resolve these overlapping signals without the need to redo the sequencing. Does anyone have suggestions or insights on how to develop an algorithm for this purpose, specifically in the context of a PCR segment? Alternatively, if such tools already exist, could you please point me in the direction of existing software or resources that can help with this? Any guidance on signal separation, deconvolution, quality filtering, alignment correction, and error correction would be greatly appreciated. Thank you!