Hi there,
I wanted to use the proteasix prediction tool - http://proteasix.cs.man.ac.uk/pred.html# - but it doesn't seem to be working at the moment.
I wanted to input protein UniProt IDs with corresponding start and stop amino acid positions of peptides within the full-length protein. I wanted the prediction tool to tell me, from this information, which proteases were most likely to have cleaved the peptide sequence from the original protein. I have around 800 peptides so this is difficult to do by hand.
I wondered if anyone could suggest an alternative to proteasix that I could try that might do the same thing?
Many thanks!
Have you considered emailing the address listed? Sometime labs put these tools up and once a grad student/post-doc leaves the PI may forget their existence. You may also want to directly email the PI to see if they care about fixing the issue.
Thanks for the suggestion! Yes I did email them a while ago and no reply just yet, emailing the PI is probably a good idea