BLAST database error

Question

[Mero]

Hello, For my project I downloaded blast+ executables and also a database using this:

 perl ../ncbi-blast-2.14.1+/bin/update_blastdb.pl --passive --decompress nt_euk.01

Then I tried to extract fasta sequence from this nt_euk.01 database using this command:

blastdbcmd -entry all -db nt_euk.01 -out nt_euk1.fasta

But it gave me this error:

Error: mdb_env_open: No such file or directory

I made sure to check my blast and blastdb paths and it seems there is no problem there. And also blastdbcmd command has worked on 16S_ribosomal_RNA database. I couldn't understand what I am doing wrong. I'd appreciate some guidance. Thanks in advance!

Answer 1

[GenoMax]

You can't download just one of the files for nt_euk (looks like there are 76 files). You will need to download all and then uncompress them in a directory to use nt_euk..