missing region in the process of annotation
0
0
Entering edit mode
5 months ago
Aki ▴ 20

Hi. I am analyzing TCGA methylation data from TCGAbiolinks and I faced one problem during annotation process with annotatr. This TCGA data has covered a gene in the chromosome 19, but annotated result did not contain one region in chromosome 19. I used the opensea region probe for compartment analysis and there is no opensea probe for these genes in missing region. The genes are covered by only Island, N_Shore, and S_Shore region probe. Is that because, I couldn't observe the annotation result of the gene? I used following script. Thank you for your help.

library(minfi)
compartments_result <- compartments(GRset, chr = paste0("chr", 19), what = "OpenSea", resolution = 100 * 1000)

library(annotatr)
annots = 'hg19_basicgenes'
annotations = build_annotations(genome = 'hg19', annotations = annots)

AB_annotated_results <- annotate_regions(regions = compartments_result,
                                         annotations = annotations,
                                         ignore.strand = TRUE,
                                         quiet = FALSE)
annotatr minfi • 212 views
ADD COMMENT

Login before adding your answer.

Traffic: 1797 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6