I would like your advice on how to normalize, a Log2 normalized values of transcripts from DEGs flowerets in eight consecutive stages of development across, each stage has 3 biological replicates with their reads data.
I know they're logged, but my supervisor suggested we scale(scale=T, center=T) is this the best way to scale them? I tried it but the heatmap with scaling and without is nearly the same.
I'm planning to do PCA &Correlation on the data and further biostat analysis. Any advice is appreciated.