Do you recommend to use t to rank gene or log2FC or sign(log2FC) * -log10(Pvalue) to rank gene?
The fgsea use t to rank the gene. fgsea rank gene Rscript
While when looking the distribution of the DEGs, t is similar to log2FC, but different from the others like Pvalue, adj.Pvalue, etc.
Aslo, when looking at the results from GSEA, it seems Pvalue is more mentationed, while not using adjusted-P value?