Entering edit mode
7 months ago
becky.reese
•
0
I am having trouble generating an index with STAR. It always kills at "inserting junctions into the genome indices". I have 32GB of RAM and I tried limiting the RAM used, but that didn't seem to work. Are there other flags I can try before I resort to buying another stick of RAM?
STAR --runThreadN 12 --runMode genomeGenerate -genomeDir ./ --genomeFastaFiles Homo_sapiens.GRCh38.dna.primary_assembly.fa --sjdbGTFfile Homo_sapiens.GRCh38.110.gtf --sjdbOverhang 149 --limitGenomeGenerateRAM 28000000000
I can figure out how to upload the log file if that is helpful
Thanks!!
This worked!! Thank you so much!! And thank you for the tip on the RAM usage command as well