Entering edit mode
4 months ago
analyst
▴
50
Dear scientists,
I have to perform imputation of my variants. I am following loimpute for imputation that requires input variants in mpileup format and reference phased vcf file through this command:
> loimpute -i file.mp.gz -h refpanel-phased.vcf.gz -o outputfile
To run above command I prepared mpileup files through samtools command:
> samtools mpileup file.bam | gzip -c > file.mp.gz
However I am not sure how to make this refpanel-phased vcf file for wheat.
Looking forward to your valuable suggestions.
Thanks