How to do DESeq2 on Kallisto Data
1
0
Entering edit mode
3 months ago
Aaliya • 0

Hello! I am doing DESeq2 for the first time. A bit of background: I am downloading the already public data available from ENA browser. I have been able to successfully do Kallisto on the paired reads. The output of such files in in .tsv format. I am really confused on how to proceed with DESeq2 after this? I do have the set parameters for the log2 count and probability. I have 6 samples: 3 replicates of treated condition and 3 replicates of the controlled condition.

Can someone pls hep me out, i am really lost on how to give it a start. Does anyone have a pipeline they are willing to share? It will help me a bit!

Unfolded Kallisto DESeq2 Reponse Protein • 434 views
ADD COMMENT
0
Entering edit mode

Review the DESeq2 standard workflow.

ADD REPLY
1
Entering edit mode
3 months ago
dsull ★ 6.1k

Cross-posted: https://support.bioconductor.org/p/9156404/

^And I responded there

ADD COMMENT

Login before adding your answer.

Traffic: 1409 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6