Pseudo bulking of scATAC-seq
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Entering edit mode
8 weeks ago
Manhezz • 0

Hello,

I have different cell clusters generated using Signac. i want to cal enhancers and super enhancers within a cluster of interest using ROSE algorithm(python ROSE_main.py -g HG37 -i enhancers.gff -r BAM -o Hezz -s 12500 -t 2500), using this code how do i come up with the bam file for each cluster? data type is scATAC

scATAC-seq SIgnac • 231 views
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Entering edit mode
8 weeks ago

Use sinto to split the bam into one for each cluster by feeding in the barcodes of cells for each cluster.

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