Entering edit mode
9 weeks ago
valentinapazom
▴
10
Hi everyone! I need to run popscle to perform demultiplex of scRNA-seq data. Im just beginning with tutorial data but the dsc-pileup stop with segmentation fault and without any error/informative message. This is the code that I'm using (very similar to tutorial)
./bin/popscle dsc-pileup --sam ../data/jurkat_293t_downsampled_n500_full_bam.bam --vcf ../data/jurkat_293t_exons_only.vcf.withAF.vcf.gz --out ../data/result
And the terminal output is:
[cramore dsc-pileup] -- Produce pileup of dsc-RNAseq
NOTICE [2024/03/14 15:14:39] - Finished loading 737280 droplet/cell barcodes to consider
NOTICE [2024/03/14 15:14:39] - Initializing BCF reader..
NOTICE [2024/03/14 15:14:39] - Finished identifying 2 samples to load from VCF/BCF
NOTICE [2024/03/14 15:14:39] - Initializing SAM reader..
NOTICE [2024/03/14 15:14:39] - Identified 25 overlapping chromosomes between VCF and BAM
zsh: segmentation fault ./bin/popscle dsc-pileup --sam --vcf --group-list --out
Does anyone know how I can solve this error? Maybe is important to consider that I'm using macOS