Hello does anyone know how to obtain singletons from OrthoVenn3? I did a 3 way orthology comparison of 3 different strains of Cryptosporidium (2 species, 3 strains, 3 protein fastas). When I navigate to "Table Data" I see hyperlinks for "Singletons" but when I click on them it leads to a "500 Internal Server Error" page.
I tried using a script to extract only the proteins that are not in the orthogroups, but I found a larger number of proteins not in orthogroups than the number of singletons Orthovenn3 indicates. This may be because some proteins are "Unassigned" and are not in an orthogroup but are also not singletons. My parameters are e-value 1*10-5 and inflation value 1.50. I used the OrthoFinder algorithm.
I reached out to the Orthovenn3 contact but no info yet. If anyone has experience with this and can advise that would be very helpful. Thank you!
