Seurat-DE analysis
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27 days ago
odi ▴ 10

I was wondering in differential analysis in seurat does ident.2 represent the reference level?

For example, I am interested in doing DE analysis for LC vs MC and i would like the MC to be my reference. In this example, MC is made of 2 clusters (2 and 3). Would this code be right?

LCvsMC<- FindMarkers(K, ident.1 = 4, ident.2 = c(2,3))

seurat DE • 107 views
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