PolyA in FastQC results from EM-Seq?
0
0
Entering edit mode
26 days ago
Watermelon • 0

Hello all,

I received the first EM-seq data for our samples and especially R2 have polyA sequences in the file according to FastQC results. I'm not familiar with why this could occur and the sequencing company had no answer for me. I'm hoping someone can shed a light? FastQC Adapter content from R2 of a sample

Also, if you could recommend how to trim these, I would very much appreciate it. I was thinking of using TrimGalore -a2 A{20}. Would I also need to trim R1's in the same way?

fastqc wgbs polya methylation emseq • 162 views
ADD COMMENT

Login before adding your answer.

Traffic: 2390 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6