Error when i use EVidenceModeler to do the genome annotation
1
0
Entering edit mode
9 weeks ago
peanut • 0

Dear all, When i use EVidenceModeler to do the genome annotation,it get the error:


successfully changed directories to acu.partitions/GWHBGBO00000001/GWHBGBO00000001_10890001-10990000

-reading genomic sequence.

** Processing features (+)

-finding all potential splice sites, looking for GT/GC donors and AG acceptors.
-finding all potential starts and stops. (strand: +)
-loading gene prediction evidence. (strand: +)-WARNING, skipping predType: Augustus, since not specified in weights file.
-WARNING, skipping predType: Augustus, since not specified in weights file.
-WARNING, skipping predType: Augustus, since not specified in weights file.
-WARNING, skipping predType: Augustus, since not specified in weights file.
-WARNING, skipping predType: Augustus, since not specified in weights file.
-WARNING, skipping predType: Augustus, since not specified in weights file.
-WARNING, skipping predType: GeneMark.hmm3, since not specified in weights file.
-WARNING, skipping predType: GeneMark.hmm3, since not specified in weights file.
-WARNING, skipping predType: GeneMark.hmm3, since not specified in weights file.
-WARNING, skipping predType: GeneMark.hmm3, since not specified in weights file.
-WARNING, skipping predType: GeneMark.hmm3, since not specified in weights file.

and the weights.txt is like that:

PROTEIN gth 4
TRANSCRIPT  transdecoder    8
ABINITIO_PREDICTION AUGUSTUS    1
ABINITIO_PREDICTION GeneMark.hmm    1

Can you help me ? Thank you a lot!!

EVidenceModeler annotation genome • 370 views
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1
Entering edit mode
8 weeks ago
  1. you got warnings, not errors
  2. EVM is looking for Augustus, not AUGUSTUS - case matters probably
  3. EVM is looking for GeneMark.hmm3 not GeneMark.hmm as you wrote.
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Entering edit mode

thank you so much ,i get it

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