BLASTP short sequences (<20aa) - interpreting results
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5 weeks ago
neish • 0

I am an extreme novice and doing my first bioinformatics project looking at in silico peptide vaccine design and for my final section, I needed to search around 250 peptide sequences (all <20aa) through BLASTP on the NCBI web version to identify the specificity of the peptides chosen. I have searched them through with the automatically adjusted parameters for short input sequences, however I am now struggling with the best way to interpret these results as it obviously provides a variety of results ranging from low to high E-values.

I also need to search them against homo sapiens, to ensure the peptides aren't also present in humans, but if I automatically adjust the search, it will come out with results, but again I do not know how to interpret these, as I will have to mention it came out with results which in the context of my project is bad, but the E-values are mostly very high.

Can anyone provide any advice?

blast sequence blastp alignment • 258 views
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I needed to search around 250 peptide sequences

How long are these?

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All short sequences, on average 15 amino acids each.

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