Comparison between Bracken outputs
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20 days ago
SushiRoll ▴ 120

Hey everyone,

I have been doing some taxonomic detection using Kraken2 and later species abundance estimation using Bracken. My input data are short metagenomic reads. My question is the following: If I have 2 different datasets with different read count and I want to compare the abundance in species between these two datasets, should I normalize the Bracken assigned reads to the corresponding total classified reads? I'm confused as to whether Bracken does any sort of normalisation in this sense.

Thanks for any help!

Bracken Kraken Taxonomy • 111 views
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